Case Study Optics
Advancing Next-Generation Multi-Channel qPCR Optics.
Technology
Multi-Channel Fluorescence
Detection for qPCR
Market/Application:
Point-of-Care MolecularÂ
Diagnostics
Delivering high-performance molecular diagnostics at the point of care requires optical systems that ensure accuracy, precision, and reliability — from design to deployment. As qPCR-based platforms evolve toward higher multiplexing and faster results, optical subsystems must provide exceptional sensitivity and specificity within increasingly compact and cost-sensitive designs.
Â
We developed an advanced fluorescence detection concept that met these demands — combining miniaturization, manufacturability, and high analytical performance in one scalable module.
Major Challenges
- Increase number of fluorescence detection channels
- Minimize optical cross-talk and boost sensitivity
-
Meet ambitious cost and manufacturability targets for commercial scale-up
Achieving these objectives required a careful balance between optical accuracy and precision, system compactness, and manufacturability, while ensuring robust performance for both quantitative and semi-quantitative molecular detection.
Our Solution
-
A transparent, trade-off-based development approach was used to evaluate and compare multiple optical architectures. Two rapid prototypes were created using primarily off-the-shelf components, enabling quick iteration and proof-of-principle demonstration.
The final design featured a compact, static six-channel fluorescence optics module, with scalability for additional two channels. Designed with clear cost–performance criteria, it used tightly toleranced plastic components to achieve micrometer-level optical precision in a compact form factor.
Simulation-driven modeling, tolerance analysis, and iterative in-house verification ensured optical accuracy and manufacturability. This agile process enabled rapid refinement, reduced development time, and minimized technical risk.
- The system delivers:
- High sensitivity: Ultra-low limits of detection
- High specificity: Minimal channel cross-talk
- Signal stability: Utra-stable, low variation fluorescence response
-
These performance features enable accurate quantitative and semi-quantitative detection of multiple DNA targets — essential for rapid and reliable point-of-care diagnostics across diverse pathogens.
Proven Expertise
-
Tailored design & complete ownership: A custom optics module optimized for specific performance, cost, and integration needs, with full IP retained by the customer.
-
Iterative prototyping: Multiple development rounds enabled fast adaptation, hands-on evaluation, and continuous improvement.
-
In-house verification: Rapid prototyping and testing in LRE’s dedicated optics labs and manufacturing lines accelerated development timelines.
-
Simulation-driven design: Advanced modeling and tolerance analysis ensured optical precision and minimized early-stage risk.
-
Design-for-excellence: Cost-effective assembly, testing, and manufacturability were integrated from the start.
-
Regulatory alignment: The entire process fully complied with FDA and ISO 13485 regulations.
Use Case
Point-of-Care
